Antibody Conjugation Kits
Chromalink Biotin Labeling Reagent, water soluble (MVS-B-1007-110) |
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The most advanced, intelligent and easy to use biotinylation reagent and kits. It is a better biotin.
Applications include:
Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin 345S accurately determines the degree of biotin incorporation without any of these disadvantages.This protein-labeling reagent, also available in an easy-to-use kit (B-9007-105), contains three important features that make it ideal for biotinylation: 1.A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling; 2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications; 3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems. |