Antibody Conjugation Kits

Chromalink One-Shot Antibody Biotinylation Kit (MVS-B-9007-009K)
The ChromaLink™ One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple A280 and A354 measurement determines the number of biotins per antibody molecule. Highlights: Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody Fast and easy method to biotinylate and determine the amount of labeling Label and determine the extent of biotin labeling with one reagent Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide) Nondestructive assay does not destroy biotinylated sample Applications include: Biotinylate antibodies Quantitate the extent of biotin labeling Optimize biotin-avidin complex. Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.

Chromalink One-Shot Antibody Biotinylation Kit (MVS-B-9007-009K)

The ChromaLink™ One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple A280 and A354 measurement determines the number of biotins per antibody molecule.
Highlights:

Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody
Fast and easy method to biotinylate and determine the amount of labeling
Label and determine the extent of biotin labeling with one reagent
Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
Nondestructive assay does not destroy biotinylated sample

Applications include:

Biotinylate antibodies
Quantitate the extent of biotin labeling
Optimize biotin-avidin complex.

Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.

A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.