Antibody Conjugation Kits

Chromalink Biotin Labeling Kit (MVS-B-9007-105K)
The most advanced, intelligent and easiest to use biotinylation kit with a better Biotin. The ChromaLink™ Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols and water soluble reagents, so it is safe, no organics needed. Applications include: Biotinylate antibodies or proteins Quantitate the extent of biotin labeling Optimize biotin-avidin complex. Highlights: Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram Fast and easy method to biotinylate and determine the amount of labeling Label and determine the extent of biotin labeling with one reagent Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein) Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling. A long polyethylene glycol (PEG3) spacer, that reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.

Chromalink Biotin Labeling Kit (MVS-B-9007-105K)

The most advanced, intelligent and easiest to use biotinylation kit with a better Biotin.
The ChromaLink™ Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols and water soluble reagents, so it is safe, no organics needed.
Applications include:

Biotinylate antibodies or proteins
Quantitate the extent of biotin labeling
Optimize biotin-avidin complex.

Highlights:

Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
Fast and easy method to biotinylate and determine the amount of labeling
Label and determine the extent of biotin labeling with one reagent
Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein)
Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations.

Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.

A long polyethylene glycol (PEG3) spacer, that reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.