|Rabbit polyclonal antibody to Tyrosine Hydroxylase (TH)|
|Immunohistochemical detection of Tyrosine Hydroxylase in rat brain Substantia Nigra. Brain was fixed with 4% formaldehyde and cut into 10 μm thick cryostat sections. Tissue was incubated with rabbit polyclonal antibody to Tyrosine Hydroxylase at 2 μg/mL overnight at 4°C followed by incubation with Donkey anti-rabbit Rhodamine Red conjugated secondary antibodies at 1:200.
Neuronal nuclei were counterstained with DAPI (blue)
|Unit Size:||50 µg|
|Alternative Names||Tyrosine 3-hydroxylase|
Plays a key role in the physiology of adrenergic neurons. TH catalyzes the conversion of the amino acid L-tyrosine to dihydroxyphenylalanine (DOPA), which, in turn, serves as a precursor for neurotransmiters dopamine, norepinephrine (noradrenaline) and epinephrine (adrenaline) the conversion of tyrosine to dopamine.
|Applications:||Immunohistochemistry (IHC), Immunocytochemistry (ICC), Western Blotting (WB).|
|Working Dilution for Immunofluorescence (ICC):||1– 15 µg/mL|
|Working Dilution for Immunohistochemistry (IHC):||1– 10 µg/mL|
|Working Dilution for Western Blottin (WB):||1 µg/mL|
|IHC Positive control:||Brain, spinal cord, neuroendocrine cells|
|Specificity:||Confirmed by WB.|
|Cross-reactivity:||Human; mouse; rat|
|Reconstitution:||Reconstitute in 0.05 mL of PBS (pH 7.4) to achieve an antibody concentration of 1000 µg/mL. Centrifuge to remove any insoluble material.|
|Storage / Stability:||At least 12 months after purchase at 2 - 4°C. After reconstitution, aliquot and store at -20°C for a higher stability and at 4°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.|