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Antibody Conjugation Kits



Chromalink Biotin Labeling Reagent, water soluble (MVS-B-1007-105)

The most advanced, intelligent and easy to use biotinylation reagent and kits. It is a better biotin.
The new ChromaLink Biotin Labeling Reagent is faster and easier to quantitate than the traditional and destructive HABA:Avidin displacement biotin measurement method.

  • Sufficient reagent to label up to 100 milligrams of protein
  • Fast and easy method to biotinylate and determine the amount of labeling
  • Label and determine the extent of biotin labeling with one reagent
  • Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)

Applications include:

  • Biotinylate antibodies, proteins or peptides
  • Quantitate the extent of biotin labeling
  • Optimize biotin-avidin complexes

Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin 345S accurately determines the degree of biotin incorporation without any of these disadvantages.This protein-labeling reagent, also available in an easy-to-use kit (B-9007-105), contains three important features that make it ideal for biotinylation: 1.A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling; 2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications; 3. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.





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