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Antibody Conjugation Kits



Chromalink One-Shot Antibody Biotinylation Kit (MVS-B-9007-009K)

The ChromaLink™ One-Shot Antibody Labeling Kit offers the complete solution to the quantitative biotinylation of 100 ug of antibody . The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation. A simple A280 and A354 measurement determines the number of biotins per antibody molecule.

  • Complete Kit, contains sufficient material to biotinylate 100 ug of Antibody
  • Fast and easy method to biotinylate and determine the amount of labeling
  • Label and determine the extent of biotin labeling with one reagent
  • Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein/peptide)
  • Nondestructive assay does not destroy biotinylated sample

Applications include:

  • Biotinylate antibodies
  • Quantitate the extent of biotin labeling
  • Optimize biotin-avidin complex.

Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages.  ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.

  1. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
  2. An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.





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