The most advanced, intelligent and easiest to use biotinylation kit with a better Biotin.
The ChromaLink™ Biotin Labeling Kit has everything you need and is faster and easier for labeling and measuring the amount of bound biotin than old traditional HABA:Avidin displacement measurement method. The Kit also has simple protocols and water soluble reagents, so it is safe, no organics needed.
- Biotinylate antibodies or proteins
- Quantitate the extent of biotin labeling
- Optimize biotin-avidin complex.
- Complete Kit, contains sufficient material to biotinylate five samples of 25 micrograms to 1 milligram
- Fast and easy method to biotinylate and determine the amount of labeling
- Label and determine the extent of biotin labeling with one reagent
- Simple calculations based on UV absorbance estimates total biotin incorporation (moles of biotin per mole of protein)
- Nondestructive assay does not destroy biotinylated sample Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations.
Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
- A long polyethylene glycol (PEG3) spacer, that reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications.
- An aromatic N-hydroxysuccinimide (NHS) ester reactive group, known to efficiently couple to amines, such as the e-amino group of lysine (K) or the a-terminus of the protein in aqueous systems.