The Fluorescein Antibody Labeling Kit is designed to label a microscale quantity of antibody (100 µg) with 3 to 5 fluorescein molecules per antibody. The kit contains sufficient reagents to perform two labeling reactions, 100 µg of antibody per reaction. Fluorescein-labeled antibodies can be used for standard immunofluorescent staining and imaging of cells or tissues. This kit contains all the necessary components to label and purify an antibody in about 90 minutes.
Fluorescein is a very popular fluorphore used in a wide variety of applications, with its absorption maximum at 494 nm and emission maximum at 518 nm (in water). The major derivative is Fluorescein isothiocyanate (FITC).
The Fluorescein Antibody Labeling Kit is designed to perform two conjugations of 100 µg of any user-supplied antibody with pre-activated high-fluorescent fluorescein. Any suitably pure monoclonal or polyclonal antibody can be conjugated to fluorescein. Best of all, our kits are specifically designed for researchers with little or no conjugation experience.
The features and benefits of this kit include:
- Fluorescein-IgG conjugates in just over 90 minutes
- Includes columns to easily deliver purified conjugates
- Gentle chemistry: Leaves antibodies intact – No reducing reagents
- Easy-to-use protocols and calculators
- Compatible with western, ELISA, flow cytometry, and IHC/ICC applications
This is the only kit on the market that can guarantee 100% conversion of antibody to conjugate, a purified conjugate free of any unlabeled antibody and a high final yield of >70%.