Now with TurboLink Catalyst Buffer
Includes everything needed to make up to 1mg of great PE-Antibody conjugates. Includes purification, saves time, no experience needed.
HydraLink™ conjugation technology allows you to more easily and efficiently prepare R-PE-antibody conjugates. The kit includes all the reagents and buffers needed to perform the two step conjugation to label up to 1mg of antibody. Our R-PE_antibody bioconjugation technology is superior to the maleimido/thiol-based method as it is: More efficient: Greater than 95% of antibody is converted to conjugate.More easily purified: In most cases, the percent conversion of free antibody to conjugate is 100%, leaving no unconjugated antibody. Therefore it is only necessary to purify out the excess R-PE to obtain a purified conjugate. In many applications even, purification is not necessary. Controllable: the level of polymerization, and therefore the brightness of the conjugate, can be controlled by adjusting the level of HyNic-modification on the antibody. In flow cytometry applications, heterodimer product is required and preparation of this construct can be easily optimized using this technology. Gentle on antibodies: other conjugation methods expose thiols on antibodies by DTT reduction of disulfide bonds, which cleaves the antibody into a variety of species. Our technology, however, gently incorporates HyNic moieties on the intact antibody.