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Si-Mag™ PG1 Kit


PCR DNA extraction and purification magnetic beads kit
This kit provides a simple, rapid and efficient method for the recovery and purification of DNA directly from PCR products (100 bp to 50 kb) with typical recovery efficiency up to 85%. The resulting product can be directly used for sequencing, restriction digestion, or PCR and other downstream experiments. In addition, the kit can be used to concentrate DNA.
The kit will work with a 96 well round bottom plates if a special magnetic frame is used. The kit
can also be used with a variety of automatic nucleic acid extraction instruments and workstation.

Catalog #

Si-Mag-PG1

Precautions

  • Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.
  • Avoid freeze/thaw cycles and centrifugation which could damage the beads.
  • Vortex samples for about 10 seconds before adding magnetic beads.
  • Vortex beads for about 10 seconds and mix them well with DNA containing material to ensure best performance.
  • Elute DNA from the beads completely.

Kit Components included

  1. Si-Mag magnetic beads 5 mL
  2. PCR DNA binding buffer 9 mL (Add 6 mL of Isopropanol before use)
  3. Elution Buffer 4 mL

Materials needed but not provided with the kit

  • 80% Ethanol in water.
  • Si-Mag Magnet (sold separately) or other magnetic racks compatible with vials used.
  • Isopropanol (ACS grade).

Storage

Magnetic beads should be stored at 2-8°C but other kit reagents need to be stored at room temperature.

Protocol

  1. Sample preparation. Add 3 volumes of PCR DNA binding solution directly to the PCR product. For example, add 120 ul of PCR DNA binding buffer to a 40 ul of PCR product.

  2. Transfer all content to an Eppendorf tube, then add 50 uL of magnetic beads, mix well and incubate 3-5 min at RT. Put Eppendorf tube onto the Si-Mag magnet rack for 20 seconds. Remove supernatant by holding the magnet rack upside or by pipetting.

  3. Wash the beads with 500 uL of 80% ethanol twice.
  4. Dry the beads at 55°C for 8 min leaving the tube open. Do not over-dry the beads.

  5. Elute the DNA from beads with 35 uL of elution buffer, incubate for at least 2 min and then vortex at full speed for 1 min. Alternatively, incubation at 60°C for 2 min may improve the recovery for DNA larger than 3 kb.

  6. Remove beads by using magnet rack, pipette DNA out and transfer to a clean tube.

  7. Store purified DNA at -20°C for long-term storage.

 

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