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Si-Mag™ VD1 Kit


Viral DNA extraction and purification magnetic beads kit
This kit allows for extraction and purification of viral DNA from serum, plasma, urine, lymph, cell culture supernatants or from a variety of viral-containing fluids. Using proprietary Viral Lysis solution, viral DNA can be efficiently extracted and then purified using magnetic beads, yielding high pure viral DNA with a ratio of OD260/280 between 1.75 and 1.85. The recovered DNA size can be up to 60kb.
The kit will work with a 48 well round bottom plates if a special magnetic frame is used.
The kit can also be used with a variety of automatic nucleic acid extraction instruments or workstations.

Catalog #

Si-Mag-VD1

Precautions

  • Wear protective gloves, clothing, eye, and face protection. Wash hands thoroughly after handling.
  • Avoid freeze/thaw cycles and centrifugation which could damage the beads.
  • Proteinase K solution should be stored at -20°C.
  • Bring frozen viral samples to room temperature before extraction.
  • Vortex samples for about 10 seconds before adding magnetic beads.
  • Vortex beads for about 10 seconds and mix them well with DNA containing material to ensure best performance.
  • Elute DNA from the beads completely.

Kit Components included

  1. Si-Mag magnetic beads 10 mL
  2. Proteinase K solution 2 mL
  3. Viral Lysis solution 20 mL
  4. Wash solution 38 mL (mix with 25 mL of Isopropanol before use)
  5. Elution Buffer 20 mL

Materials needed but not provided with the kit

  • 80% Ethanol in water.
  • Si-Mag Magnet (sold separately) or other magnetic racks compatible with vials used.
  • Isopropanol (ACS grade).

Storage

Magnetic beads should be stored at 2-8°C but other kit reagents need to be stored at room temperature. Lysis solution may turn cloudy if store in th cold room and to clear it up place the bottle into a water bath at 37°C.

Protocol

  1. Preparation of sample. Add 200 uL of viral fluid sample, 200 uL of viral lysis solution, and 20 uL of Proteinase K solution into a clean Eppendorf tube. Vortex for 30 seconds then incubate for 10 min at 58°C.
  2. Add 100 uL of magnetic beads to the tube.
  3. Add 300 uL of isopropanol to the tube.
  4. Mix the tube well and incubate 5 min at room temperature.
  5. Put the Eppendorf tube onto the Si-Mag magnet rack for 20 seconds. Make sure the beads are collected at the bottom of the tube.
  6. Remove supernatant by holding the magnet rack upside down or by pipetting.
  7. Wash the beads with 600 uL of wash solution. Vortex the tube to mix well.
  8. Wash the beads with 500 uL of 80% ethanol for twice and repeat Step 5.
  9. Dry the beads at 55°C for 3-4 min leaving the tube open. Do not over-dry the beads.
  10. Elute the DNA from beads with 50-200 uL of elution buffer, incubate at 60°C for 2 min and then vortex at full speed for 1 min. At 5 min, repeat the vortexing once.
  11. Remove beads by using magnet rack, pipette DNA out and transfer to a clean tube.
  12. Store purified DNA at -20°C for long-term storage.
 

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